Recent advances in digital technologies have led to an increasing interest in the science and engineering of digital holographic microscopy (DHM). DHM allows for non-contact 3D microscopic measurements by digitally recording the object as a hologram. Using a numerical reconstruction algorithm, a computer then calculates the object image. The main application areas can be found in bio-medical (cell imaging, cell analysis) and material science applications (3D topography, surface finish analysis, MEMS characterization).
By using a diffraction-limited aspheric lens from asphericon, Kent Wallace and his team (California Institute of Technology, University of Washington, McGrill University) have developed a novel off-axis DHM that avoids the limitations of prior systems and provides a robust, compact and easy-to-use performance. The authors also show that their new design supports use in both routine laboratory settings as well as extreme environments.
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Publication: J. Kent Wallace, Stephanie Rider, Eugene Serabyn, Jonas Kühn, Kurt Liewer, Jody Deming, Gordon Showalter, Chris Lindensmith, and Jay Nadeau: Robust, compact implementation of an off-axis digital holographic microscope, Optics Express, Vol. 23, Issue 13, pp. 17367-17378, 2015, doi: 10.1364/OE.23.017367.